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Ankyron SFRP2_MOUSE Clone AO40650
Clone Information
|
Clone ?
|
AO40650 |
|
Gene
|
Sfrp2 Sarp1 Sdf5 |
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UniProtID
|
P97299 |
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Organism
|
Mus musculus (Mouse) |
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Entry Name
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SFRP2_MOUSE |
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SNR ?
|
377 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, WB |
|
Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
J774A.1 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse J774A.1 following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1 µg) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40650 binding to 0.5 picomoles of immobilised SFRP2_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.