Ankyron SEM4D_MOUSE Clone AU40184

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Price £245.00
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Clone Information

Clone ?

AU40184
Gene

Sema4d Semacl2 Semaj
UniProtID

O09126
Organism

Mus musculus (Mouse)
Entry Name

SEM4D_MOUSE
SNR ?

797
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

Mouse macrophage J774A.1 cells were stimulated with LPS overnight, fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Colorimetric western blot analysis of purified target protein (1µg - 100 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AU40184 binding to 0.5 picomoles of immobilised SEM4D_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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