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Ankyron SEM4D_HUMAN Clone DC40955
Clone Information
|
Clone ?
|
DC40955 |
|
Gene
|
SEMA4D C9orf164 CD100 SEMAJ |
|
UniProtID
|
Q92854 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
SEM4D_HUMAN |
|
SNR ?
|
109400 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IHC, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Immunohistochemical analysis of paraffin-embedded human tonsil using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Flow cytometric analysis of human lymphocytes following fixation with formaldehyde (1%) and subsequent co-incubation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DC40955 binding to 0.5 picomoles of immobilised SEM4D_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.