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Ankyron SELPL_HUMAN Clone AY40106
Ankyrons® Testing Data
Lymphocytes were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AY40106 binding to 0.5 picomoles of immobilised SELPL_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Flow cytometric analysis of human lymphocytes following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Immunohistochemical analysis of paraffin-embedded human lymph node using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1:500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Citrate buffer (pH6).
Immunohistochemical analysis of paraffin-embedded human lymph node using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1:500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).