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Ankyron SDCB1_HUMAN Clone AL40306
Clone Information
|
Clone ?
|
AL40306 |
|
Gene
|
SDCBP MDA9 SYCL |
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UniProtID
|
O00560 |
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Organism
|
Homo sapiens (Human) |
|
Entry Name
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SDCB1_HUMAN |
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SNR ?
|
6096 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, Kd, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd <10nM |
Ankyrons® Testing Data
MCF7 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of permeabilised MCF7 cells following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot of recombinant protein and lysate prepared from HCT116, using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP antibody.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AL40306 binding to 0.5 picomoles of immobilised SDCB1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AL40306 binding to SDCB1_HUMAN, of value Kd = 1.36 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.