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Ankyron SCG3_HUMAN Clone AL40294
Clone Information
|
Clone ?
|
AL40294 |
|
Gene
|
SCG3 UNQ2502/PRO5990 |
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UniProtID
|
Q8WXD2 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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SCG3_HUMAN |
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SNR ?
|
11 |
|
Product Type
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Monomer |
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Ankyron Applications
|
IF, ELISA, WB |
|
Pack Size
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100ug |
|
Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HepG2 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Colorimetric western blot analysis of lysates from HepG2 cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AL40294 binding to 0.5 picomoles of immobilised SCG3_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.