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Ankyron REG3G_HUMAN Clone AW40814
Clone Information
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Clone ?
|
AW40814 |
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Gene
|
REG3G PAP1B UNQ429/PRO162 |
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UniProtID
|
Q6UW15 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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REG3G_HUMAN |
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SNR ?
|
2272 |
|
Product Type
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Monomer |
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Ankyron Applications
|
IF, IHC, ELISA |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
MCF7 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human pancreas using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 10 min.
Immunohistochemical analysis of paraffin-embedded human pancreas using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 10 min.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AW40814 binding to 0.5 picomoles of immobilised REG3G_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.