Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CO40696 binding to 0.5 picomoles of immobilised RAF1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
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