Ankyron RAB1A_HUMAN Clone ED30321

Product Type
Pack Size
Select Label Clear All
Price US$307.00
Quantity

Clone Information

Clone ?

ED30321
Gene

RAB1A RAB1
UniProtID

P62820
Organism

Homo sapiens (Human)
Entry Name

RAB1A_HUMAN
SNR ?

204
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His
Ankyron Kd

Kd Available

Ankyrons® Testing Data

U-87 MG cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® ED30321 binding to 0.5 picomoles of immobilised RAB1A_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® ED30321 binding to RAB1A_HUMAN, of value Kd = 920.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

Purchase With