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Ankyron Q171I8_AEDAE Clone BT40178
Ankyrons® Testing Data
Confocal microscopy of Aag2-AF2 Aedes aegypti cells and Aag2-AF256 cells (a CRISPR knockout cell line with a deletion in Rel2), stained with V5-tagged Ankyron, then mouse anti-V5 antibody followed by fluorescent anti-mouse antibody for detection (grey scale), with nuclei counterstained with DAPI (blue). Lower panels, secondary antibody alone. Data are courtesy of Kevin Maringer, Pirbright Institute.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BT40178 binding to 0.5 picomoles of immobilised Q171I8_AEDAE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® BT40178 binding to Q171I8_AEDAE, of value Kd = 91.5 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.