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Ankyron PTPRC_MOUSE Clone AS40709
Clone Information
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Clone ?
|
AS40709 |
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Gene
|
Ptprc Ly-5 |
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UniProtID
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P06800 |
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Organism
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Mus musculus (Mouse) |
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Entry Name
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PTPRC_MOUSE |
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SNR ?
|
10964 |
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Product Type
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Monomer |
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Ankyron Applications
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F, IF, ELISA, WB |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse splenocytes following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AS40709 binding to 0.5 picomoles of immobilised PTPRC_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.