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Ankyron PIEZ1_HUMAN Clone DR40110
Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of Piezo-1 knock-out HEK293 cells, following staining with preconjugated Ankyron and anti-V5 APC. Red histogram represents Piezo-1 levels following rescue of Piezo-1 expression, orange histogram is Piezo-1 levels without rescue and grey histogram is staining using V5 APC alone (no Ankyron) of rescued cells. Data courtesy of Dr. Stefan Düsterhöft, University Hospital Aachen.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DR40110 binding to 0.5 picomoles of immobilised PIEZ1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.