Ankyron PGFRB_HUMAN Clone AE50235

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Clone Information

Clone ?

AE50235
Gene

PDGFRB PDGFR PDGFR1
UniProtID

P09619
Organism

Homo sapiens (Human)
Entry Name

PGFRB_HUMAN
SNR ?

491
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His, Biotin (V5, His), FLAG (V5, His), Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His)

Ankyrons® Testing Data

MCF-7 were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunofluorescence microscopy of human cardiac pericytes following overnight staining with Ankyron preconjugated with anti-V5 APC labeled Flurotag. Nuclei counterstained with DAPI. Inset panel, anti-V5 Flurotag APC alone. Data courtesy of Dr. Rahme Safakli, University of Surrey.
Immunohistochemical analysis of paraffin-embedded human kidney using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE50235 binding to 0.5 picomoles of immobilised PGFRB_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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