Ankyron PGFRA_HUMAN Clone AE40220

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Price £245.00
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Clone Information

Clone ?

AE40220
Gene

PDGFRA PDGFR2 RHEPDGFRA
UniProtID

P16234
Organism

Homo sapiens (Human)
Entry Name

PGFRA_HUMAN
SNR ?

4188
Product Type

Monomer
Ankyron Applications
F, IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)

Ankyrons® Testing Data

MCF-7 were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of human MCF7 cells following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AE40220 binding to 0.5 picomoles of immobilised PGFRA_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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