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Ankyron PECA1_HUMAN Clone AC40115
Clone Information
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Clone ?
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AC40115 |
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Gene
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PECAM1 |
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UniProtID
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P16284 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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PECA1_HUMAN |
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SNR ?
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238 |
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Product Type
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Monomer |
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Ankyron Applications
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|
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HUVEC were fixed in formaldehyde (4 %), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
HUVEC were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
HUVEC were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of HUVEC following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of HUVEC following fixation in formaldehyde and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of HUVECs following fixation with formaldehyde (1%) and subsequent co-incubation of 0.025 μg Ankyron with anti-V5 APC(red). Negative control, anti-V5 APC only.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AC40115 binding to 0.5 picomoles of immobilised PECA1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
CD31+ endothelial cells were selected by magnetic beads, following labeling with biotinylated Ankyron (2 µg, 15 min) and streptavidin microbeads ( 1 in 10, 15 min) and separation on a magnetic column. Over 96 % purity was achieved (comparable to that obtained following sorting using an antibody), with pericytes (red) visible only in the CD31-negative flow-through cells. Data courtesy of Natalie King and Dr. Paul Holloway.