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Ankyron PDK1_HUMAN Clone CD30221
Clone Information
|
Clone ?
|
CD30221 |
|
Gene
|
PDK1 PDHK1 |
|
UniProtID
|
Q15118 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
PDK1_HUMAN |
|
SNR ?
|
347 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA, Kd, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Colorimetric western blot analysis of purified target protein (1 µg) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CD30221 binding to 0.5 picomoles of immobilised PDK1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CD30221 binding to PDK1_HUMAN, of value Kd = 25.7 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.