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Ankyron PDE4D_HUMAN Clone DT40258
Clone Information
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Clone ?
|
DT40258 |
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Gene
|
PDE4D DPDE3 |
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UniProtID
|
Q08499 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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PDE4D_HUMAN |
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SNR ?
|
27 |
|
Product Type
|
Monomer |
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Ankyron Applications
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IF, IHC, ELISA, Kd |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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|
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Ankyron Kd
|
Kd Available |
Ankyrons® Testing Data
HeLa were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded placenta using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DT40258 binding to 0.5 picomoles of immobilised PDE4D_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® DT40258 binding to PDE4D_HUMAN, of value Kd = 164.0 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.