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Ankyron PD1L2_HUMAN Clone AB30225
Ankyrons® Testing Data
Flow cytometric analysis of DCs following fixation with formaldehyde (1%) and subsequent co-incubation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 apc only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB30225 binding to 0.5 picomoles of immobilised PD1L2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AB30225 binding to PD1L2_HUMAN, of value Kd = 2.65 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.