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Ankyron PARP1_BOVIN Clone FY40140
Clone Information
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Clone ?
|
FY40140 |
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Gene
|
PARP1 ADPRT |
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UniProtID
|
P18493 |
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Organism
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Bos taurus (Bovine) |
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Entry Name
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PARP1_BOVIN |
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SNR ?
|
2996 |
|
Product Type
|
Monomer |
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Ankyron Applications
|
F, IF, ELISA |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
MDBK kidney epithelial cells from cattle were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
MDBK kidney epithelial cells from cattle were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Cells were imaged by super-resolution confocal microscopy to reveal expression of PARP-1 in the nucleus. Magnfication: 100X
Flow cytometric analysis of MDBK kidney epithelial cells from cattle following permeabilisation with Cytofix/Cytoperm and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® FY40140 binding to 0.5 picomoles of immobilised PARP1_BOVIN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.