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Ankyron NUCL_HUMAN Clone FN40366
Clone Information
|
Clone ?
|
FN40366 |
|
Gene
|
NCL |
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UniProtID
|
P19338 |
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Organism
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Homo sapiens (Human) |
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Entry Name
|
NUCL_HUMAN |
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SNR ?
|
323 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, WB |
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Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
HeLa cells were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
HeLa cells were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Flow cytometric analysis of HeLa following permeabilisation with Foxp3/transcription factor staining buffer and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® FN40366 binding to 0.5 picomoles of immobilised NUCL_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.