Ankyron NUCL_HUMAN Clone FN40358

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Price £245.00
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Clone Information

Clone ?

FN40358
Gene

NCL
UniProtID

P19338
Organism

Homo sapiens (Human)
Entry Name

NUCL_HUMAN
SNR ?

4010
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

HeLa cells were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® FN40358 binding to 0.5 picomoles of immobilised NUCL_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).

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