Ankyron NECT2_MOUSE Clone AO40343

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Clone Information

Clone ?

AO40343
Gene

Nectin2 Mph Pvr Pvrl2 Pvs
UniProtID

P32507
Organism

Mus musculus (Mouse)
Entry Name

NECT2_MOUSE
SNR ?

342
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Flow cytometric analysis of J774A.1 mouse macrophage cells following staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of J774A.1 mouse macrophage cells following fixation in formaldehyde and staining with pre-conjugated Ankyron (0.33 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40343 binding to 0.5 picomoles of immobilised NECT2_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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