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Ankyron NECT1_HUMAN Clone AO40212
Clone Information
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Clone ?
|
AO40212 |
|
Gene
|
NECTIN1 HVEC PRR1 PVRL1 |
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UniProtID
|
Q15223 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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NECT1_HUMAN |
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SNR ?
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2580400 |
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Product Type
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Monomer |
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Ankyron Applications
|
IF, ELISA, WB |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Lymphocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Colorimetric western blot analysis of purified target protein (1µg - 100 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40212 binding to 0.5 picomoles of immobilised NECT1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.