Ankyron MUC18_MOUSE Clone AU41118

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Clone Information

Clone ?

AU41118
Gene

MCAM MUC18
UniProtID

Q8R2Y2
Organism

Mus musculus (Mouse)
Entry Name

MUC18_MOUSE
SNR ?

177
Product Type

Monomer
Ankyron Applications

, ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

J774A.1 mouse macrophage were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Whole mount immunostaining of unfixed mouse ear tissue using 5 μg V5-tagged Ankyron and 8 μL anti-V5 APC Fluorotag for Ankyron.
Whole mount immunostaining of unfixed mouse ear tissue using 5 μg V5-tagged Ankyron and 8 μL anti-V5 APC Fluorotag for Ankyron.
Flow cytometric analysis of mouse J774A.1 following staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Flow cytometric analysis of mouse J774A.1 following fixation in formaldehye and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AU41118 binding to 0.5 picomoles of immobilised MUC18_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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