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Ankyron MUC18_HUMAN Clone CC30474
Clone Information
|
Clone ?
|
CC30474 |
|
Gene
|
MCAM MUC18 |
|
UniProtID
|
P43121 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
MUC18_HUMAN |
|
SNR ?
|
4329 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
HUVEC were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of HUVECs following incubation with preconjugated Ankyron ( 0.1 μg) and anti-V5 APC (red). Negative control, anti-V5 APC only.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) and lysates from HUVEC cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CC30474 binding to 0.5 picomoles of immobilised MUC18_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.