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Ankyron MSRE_MOUSE Clone BL30125
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of mouse RAW264.7 cells following incubation with preconjugated Ankyron and anti-V5 APC (red). Negative control, anti-V5 APC only. Data courtesy of Victoria Richardson and Subhankar Mukhopadhyay, Kings College London.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BL30125 binding to 0.5 picomoles of immobilised MSRE_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.