Ankyron MSH2_HUMAN Clone CK30169

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Price £245.00
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Clone Information

Clone ?

CK30169
Gene

MSH2
UniProtID

P43246
Organism

Homo sapiens (Human)
Entry Name

MSH2_HUMAN
SNR ?

85
Product Type

Monomer
Ankyron Applications

, , , ,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

HeLa were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human colon using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9), 40 min.
Flow cytometric analysis of HeLa cells following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of lysates from HCT116 cells using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CK30169 binding to 0.5 picomoles of immobilised MSH2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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