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Ankyron MK14_MOUSE Clone BC40235
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded mouse spleen using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 1000), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BC40235 binding to 0.5 picomoles of immobilised MK14_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.