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Ankyron MET_MOUSE Clone AU40528
Clone Information
|
Clone ?
|
AU40528 |
|
Gene
|
Met |
|
UniProtID
|
P16056 |
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Organism
|
Mus musculus (Mouse) |
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Entry Name
|
MET_MOUSE |
|
SNR ?
|
2477 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, ELISA, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
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Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
Flow cytometric analysis of mouse J774A.1 following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AU40528 binding to 0.5 picomoles of immobilised MET_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® AU40528 binding to MET_MOUSE, of value Kd = 43.1 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.