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Ankyron MARCO_MOUSE Clone DH40294
Ankyrons® Testing Data
Flow cytometric analysis of mouse RAW264.7 cells following incubation with preconjugated Ankyron and anti-V5 APC (red). Negative control, anti-V5 APC only. Data courtesy of Victoria Richardson and Subhankar Mukhopadhyay, Kings College London.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DH40294 binding to 0.5 picomoles of immobilised MARCO_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® DH40294 binding to MARCO_MOUSE, of value Kd = 57.3 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.