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Ankyron MAPK3_HUMAN Clone DP41087
Clone Information
|
Clone ?
|
DP41087 |
|
Gene
|
MAPKAPK3 |
|
UniProtID
|
Q16644 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
MAPK3_HUMAN |
|
SNR ?
|
2512 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
HepG2 cells were fixed in formaldehyde (1%), permeabilised with 0.5% Triton X-100 then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue).
Flow cytometric analysis of human HepG2 cells following permeabilisation with Foxp3/transcription factor staining buffer and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DP41087 binding to 0.5 picomoles of immobilised MAPK3_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.