Ankyron LYVE1_MOUSE Clone CJ30442

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Price £245.00
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Clone Information

Clone ?

CJ30442

Gene

Lyve1 Crsbp1 Xlkd1

UniProtID

Q8BHC0

Organism

Mus musculus (Mouse)

Entry Name

LYVE1_MOUSE

SNR ?

1867300

Product Type

Monomer

Ankyron Applications

, ,

Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CJ30442 binding to 0.5 picomoles of immobilised LYVE1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CJ30442 binding to LYVE1_MOUSE, of value Kd = 49.8 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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