Your basket is currently empty!
Ankyron LYVE1_MOUSE Clone CJ30442
Clone Information
|
Clone ?
|
CJ30442 |
|
Gene
|
Lyve1 Crsbp1 Xlkd1 |
|
UniProtID
|
Q8BHC0 |
|
Organism
|
Mus musculus (Mouse) |
|
Entry Name
|
LYVE1_MOUSE |
|
SNR ?
|
1867300 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA, Kd, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
|
|
Ankyron Kd
|
Kd <100nM |
Ankyrons® Testing Data
Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Mouse ear dermis was fixed in formaldehyde (1 %) overnight then stained with V5-tagged Ankyron (5 µg) and anti-V5 APC. Image captured by epifluorescence microscope, with Ankyron staining lymphatic vessels (cyan), in particular the overlapping junctions between endothelial cells.
Mouse ear dermis was fixed in formaldehyde (1 %) then stained with V5-tagged Ankyron that targets LYVE1_MOUSE (clone CJ30442) preconjugated with anti-V5 APC. Image captured by Nikon confocal microscope with Galvano scanning and NSPARC detection, showing maximum intensity projections. Ankyron staining of lymphatic vessels (grey-scale), in particular of the overlapping junctions between endothelial cells.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CJ30442 binding to 0.5 picomoles of immobilised LYVE1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® CJ30442 binding to LYVE1_MOUSE, of value Kd = 49.8 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.