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Ankyron LYAM2_HUMAN Clone DJ40171
Ankyrons® Testing Data
HUVEC were cultured for 6 hours in either the presence or absence of the proinflammatory cytokine TNF-α, fixed in formaldehyde (1%) then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DJ40171 binding to 0.5 picomoles of immobilised LYAM2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.