Ankyron LYAM2_HUMAN Clone DJ40151

HUVEC were cultured for 6 hours in either the presence or absence of the proinflammatory cytokine TNF-α, then fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.

HUVEC were cultured for 6 hours in either the presence or absence of the proinflammatory cytokine TNF-α, then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.

Flow cytometric analysis of HUVEC, either resting (blue) or stimulated for 4 - 6 h with TNFα (red) and stained with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).

Flow cytometric analysis of HUVEC, either resting (blue) or stimulated for 4 - 6 h with TNFα (red), then fixed in formaldehyde (1 %) and stained with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).

Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DJ40151 binding to 0.5 picomoles of immobilised LYAM2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.