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Ankyron LYAM1_HUMAN Clone AB40565
Clone Information
|
Clone ?
|
AB40565 |
|
Gene
|
SELL LNHR LYAM1 |
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UniProtID
|
P14151 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
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LYAM1_HUMAN |
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SNR ?
|
40989 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, IHC, ELISA |
|
Pack Size
|
100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human tonsil using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Lymphocytes from human PBMCs were cultured for 24 h in either the absence (unstimulated, blue) or presence of PHA (stimulated, red), then fixed in formaldehyde (1 %) and stained with 0.08 µg of Ankyron and anti-V5 APC conjugated antibody, applied simultaneously. Negative control: secondary antibody alone with no Ankyron present (grey)
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40565 binding to 0.5 picomoles of immobilised LYAM1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.