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Ankyron L1CAM_HUMAN Clone AO40902
Clone Information
|
Clone ?
|
AO40902 |
|
Gene
|
L1CAM CAML1 MIC5 |
|
UniProtID
|
P32004 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
L1CAM_HUMAN |
|
SNR ?
|
2917 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
HUVEC were stimulated with TNF-α (0.4 µl/ml) for 3 hours, fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
HeLa cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AO40902 binding to 0.5 picomoles of immobilised L1CAM_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.