Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BY30228 binding to 0.5 picomoles of immobilised JAM1_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.