Ankyron IL4_HUMAN Clone AA40202

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Price £245.00
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Clone Information

Clone ?

AA40202
Gene

IL4
UniProtID

P05112
Organism

Homo sapiens (Human)
Entry Name

IL4_HUMAN
SNR ?

31651
Product Type

Monomer
Ankyron Applications
IF, ELISA
Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His
Biotin (V5, His)
FLAG (V5, His)
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)

Ankyrons® Testing Data

Lymphocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AA40202 binding to 0.5 picomoles of immobilised IL4_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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