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Ankyron IL4_HUMAN Clone 01S20133
Clone Information
|
Clone ?
|
01S20133 |
|
Gene
|
IL4 |
|
UniProtID
|
P05112 |
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Organism
|
Homo sapiens (Human) |
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Entry Name
|
IL4_HUMAN |
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SNR ?
|
5507 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, IHC, ELISA |
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Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Lymphocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Lymphocytes were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Immunohistochemical analysis of paraffin-embedded human tonsil using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica EDTA buffer (pH9), 40 min.
Flow cytometric analysis of human lymphocytes, either resting (orange) or following stimulation (red) for 5 h with Cell Stimulation Cocktail (phorbol 12-myristate 13-acetate and ionomycin, with protein transport inhibitors brefeldin A and monensin), then permeabilised with Cytofix/Cytoperm and stained with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative controls, anti-V5 APC only on unstimulated (light grey) and stimulated (dark grey) cells.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® 01S20133 binding to 0.5 picomoles of immobilised IL4_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.