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Ankyron IL37_HUMAN Clone BA40520
Clone Information
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Clone ?
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BA40520 |
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Gene
|
IL37 FIL1Z IL1F7 IL1H4 IL1RP1 |
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UniProtID
|
Q9NZH6 |
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Organism
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Homo sapiens (Human) |
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Entry Name
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IL37_HUMAN |
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SNR ?
|
272 |
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Product Type
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Monomer |
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Ankyron Applications
|
F, IF, ELISA |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Monocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Monocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Flow cytometric analysis of monocytes following permeabilisation with Cytofix/Cytoperm buffer and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BA40520 binding to 0.5 picomoles of immobilised IL37_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.