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Ankyron IL2RG_MOUSE Clone AU40867
Ankyrons® Testing Data
Flow cytometric analysis of J774A.1 following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Colorimetric western blot analysis of purified target protein (1 µg) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AU40867 binding to 0.5 picomoles of immobilised IL2RG_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.