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Ankyron IL2RG_MOUSE Clone AU40868
Clone Information
|
Clone ?
|
AU40868 |
|
Gene
|
IL2RG |
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UniProtID
|
P34902 |
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Organism
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Mus musculus (Mouse) |
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Entry Name
|
IL2RG_MOUSE |
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SNR ?
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630 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, IHC, ELISA |
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Pack Size
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100ug |
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Label ?
Clones are also labelled with a His tag by default
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Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with Alphalux 488 (green) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 Alphalux 488 antibody alone.
Immunohistochemical analysis of frozen mouse spleen using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple).
Flow cytometric analysis of mouse J774A.1 following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AU40868 binding to 0.5 picomoles of immobilised IL2RG_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.