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Ankyron IL2_HUMAN Clone BC40343
Clone Information
|
Clone ?
|
BC40343 |
|
Gene
|
IL2 |
|
UniProtID
|
P60568 |
|
Organism
|
Homo sapiens (Human) |
|
Entry Name
|
IL2_HUMAN |
|
SNR ?
|
450500 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
IF, ELISA |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Lymphocytes stimulated for 5 hours with a cocktail of PMA, ionomycin, brefeldin A and monensin were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Lymphocytes stimulated for 5 hours with a cocktail of PMA, ionomycin, brefeldin A and monensin were stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BC40343 binding to 0.5 picomoles of immobilised IL2_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.