Ankyron IL1A_MOUSE Clone EE30169

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Price £245.00
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Clone Information

Clone ?

EE30169
Gene

Il1a
UniProtID

P01582
Organism

Mus musculus (Mouse)
Entry Name

IL1A_MOUSE
SNR ?

414
Product Type

Monomer
Ankyron Applications

,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His), Biotin (V5, His), FLAG (V5, His), V5, His

Ankyrons® Testing Data

J774A.1 mouse macrophage cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® EE30169 binding to 0.5 picomoles of immobilised IL1A_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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