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Ankyron IL18_HUMAN Clone BT40330
Ankyrons® Testing Data
Monocytes were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BT40330 binding to 0.5 picomoles of immobilised IL18_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.