Ankyron IL10_HUMAN Clone AB40107

Dendritic cells were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5% saponin), counterstaining nuclei with DAPI (blue).

PBMCs-derived Dendritic cells were stimulated overnight with LPS, fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.

Flow cytometric analysis of dendritic cells, either unstimulated (red) or stimulated overnight with LPS (orange), then permeabilised with Cytofix/Cytoperm buffer and stained with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC. Negative control, anti-V5 APC only (grey).

Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AB40107 binding to 0.5 picomoles of immobilised IL10_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.