Ankyron IGF1R_HUMAN Clone BX40156

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Clone Information

Clone ?

BX40156
Gene

IGF1R
UniProtID

P08069
Organism

Homo sapiens (Human)
Entry Name

IGF1R_HUMAN
SNR ?

368
Product Type

Monomer
Ankyron Applications

,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default
V5, His, Biotin (V5, His), FLAG (V5, His), Alphalux 405 (V5, His), Alphalux 488 (V5, His), Alphalux 532 (V5, His), Alphalux 555 (V5, His), Alphalux 647 (V5, His), Alphalux 680 (V5, His)

Ankyrons® Testing Data

HCT116 cells were fixed in formaldehyde (4%), then stained using 5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10 % human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
HUVEC were fixed in formaldehyde (4%), then stained using 2.5 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Image captured by epifluorescence microscopy. Inset: negative control of anti-V5 APC antibody alone.
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® BX40156 binding to 0.5 picomoles of immobilised IGF1R_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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