Ankyron I36RA_HUMAN Clone CS30464

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Price £245.00
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Clone Information

Clone ?

CS30464

Gene

IL36RN FIL1D IL1F5 IL1HY1 IL1L1 IL1RP3 UNQ1896/PRO4342

UniProtID

Q9UBH0

Organism

Homo sapiens (Human)

Entry Name

I36RA_HUMAN

SNR ?

565400

Product Type

Monomer

Ankyron Applications
IF, IHC, ELISA
Pack Size

100ug

Label ?
Clones are also labelled with a His tag by default
Alphalux 405 (V5, His)
Alphalux 488 (V5, His)
Alphalux 532 (V5, His)
Alphalux 555 (V5, His)
Alphalux 647 (V5, His)
Alphalux 680 (V5, His)
Biotin (V5, His)
FLAG (V5, His)
V5, His
HCT116 were fixed in formaldehyde (1%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of paraffin-embedded human skin using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple). Heat-mediated antigen retrieval with Leica Tris-EDTA buffer (pH9).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® CS30464 binding to 0.5 picomoles of immobilised I36RA_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.

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