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Ankyron I10R1_MOUSE Clone AK40807
Clone Information
|
Clone ?
|
AK40807 |
|
Gene
|
Il10ra Il10r |
|
UniProtID
|
Q61727 |
|
Organism
|
Mus musculus (Mouse) |
|
Entry Name
|
I10R1_MOUSE |
|
SNR ?
|
20610 |
|
Product Type
|
Monomer |
|
Ankyron Applications
|
F, IF, IHC, ELISA, WB |
|
Pack Size
|
100ug |
|
Label ?
Clones are also labelled with a His tag by default
|
Ankyrons® Testing Data
Mouse macrophage J774A.1 cells were fixed in formaldehyde (4%), then stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum), counterstaining nuclei with DAPI (blue). Inset: negative control of anti-V5 APC antibody alone.
Immunohistochemical analysis of frozen mouse spleen using 10 μg/ml V5-tagged Ankyron and anti-V5 HRP (1 in 500), detecting with DAB (brown) and counterstaining nuclei with haematoxylin (blue-purple).
Flow cytometric analysis of mouse J774A.1 following pre-conjugation of 0.4 μg of Ankyron with anti-V5 APC (red). Negative control, anti-V5 APC only.
Colorimetric western blot analysis of purified target protein (1µg - 10 ng) using 1 µg/ml V5-tagged Ankyron preconjugated with anti-V5-HRP (1 in 500).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® AK40807 binding to 0.5 picomoles of immobilised I10R1_MOUSE, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.