Ankyron HNF4A_HUMAN Clone DT40142

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Price £245.00
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Clone Information

Clone ?

DT40142
Gene

HNF4A HNF4 NR2A1 TCF14
UniProtID

P41235
Organism

Homo sapiens (Human)
Entry Name

HNF4A_HUMAN
SNR ?

851
Product Type

Monomer
Ankyron Applications

,

Pack Size

100ug
Label ?
Clones are also labelled with a His tag by default

Alphalux 405

Alphalux 488
Alphalux 532
Alphalux 555
Alphalux 647
Alphalux 680
Biotin
FLAG
V5

Ankyrons® Testing Data

Flow cytometric analysis of human HepG2 cells following permeabilization and staining with pre-conjugated Ankyron (0.4 µg) and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).
Binding assay determination of the signal to noise ratio (SNR) of V5-labeled Ankyrons® DT40142 binding to 0.5 picomoles of immobilised HNF4A_HUMAN, over a background (BG) of non-cognate target protein. The Ankyron was pre-conjugated with the ProImmune Anti-V5 HRP label reagent before detection with a chemiluminescence substrate.
Affinity measurement of V5-labeled Ankyrons® DT40142 binding to HNF4A_HUMAN, of value Kd = 13.8 nM, with 95% confidence bounds (CI95U, CI95L). Conjugation to ProImmune Anti-V5 HRP label reagent allows detection in Solution Equilibrium Titration immunoassay with a chemiluminescent substrate.

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